| Method |
Sitting-drop vapor diffusion |
Sitting-drop vapor diffusion |
| Plate type |
Cryschem sitting-drop plate |
Cryschem sitting-drop plate |
| Temperature |
Room temperature (∼296 K) |
Room temperature (∼296 K) |
| Protein concentration (mg ml−1) |
36 |
42 |
| Buffer composition of protein solution |
20 mM HEPES pH 7.5 |
20 mM HEPES pH 7.5 |
| Composition of reservoir solution |
0.2 M ammonium citrate tribasic pH 7.0, 18% PEG 3350 |
1.2 M malic acid |
| Volume and ratio of drop |
1 µl protein solution and 1 µl reservoir solution |
1 µl protein solution and 1 µl reservoir solution |
| Volume of reservoir (µl) |
500 |
500 |
