Table 2. Crystallization conditions.
| Method | Sitting-drop vapor-diffusion |
| Plate type | 48-well plates |
| Temperature (K) | 289 |
| Protein concentration (mg ml−1) | 10 |
| Buffer composition of protein solution | 20 mM Tris–HCl, pH 7.5, 100 mM NaCl, 1 mM DTT, 5%(v/v) glycerol |
| Composition of reservoir solution | 0.24 M lithium sulfate, 0.1 M Tris–HCl pH 8.5, 26%(w/v) PEG 4000 |
| Volume (µl), ratio of drop | 2, 1:1 ratio of protein:reservoir solution |
| Volume of reservoir (µl) | 100 |