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. 2018 Sep 19;74(Pt 10):625–631. doi: 10.1107/S2053230X18010920

Table 2. Crystallization conditions.

Method Sitting-drop vapor-diffusion
Plate type 48-well plates
Temperature (K) 289
Protein concentration (mg ml−1) 10
Buffer composition of protein solution 20 mM Tris–HCl, pH 7.5, 100 mM NaCl, 1 mM DTT, 5%(v/v) glycerol
Composition of reservoir solution 0.24 M lithium sulfate, 0.1 M Tris–HCl pH 8.5, 26%(w/v) PEG 4000
Volume (µl), ratio of drop 2, 1:1 ratio of protein:reservoir solution
Volume of reservoir (µl) 100