Skip to main content
. 2018 Oct;188(10):2339–2355. doi: 10.1016/j.ajpath.2018.06.022

Figure 10.

Figure 10

Beclin1 and Rab7 degradation inhibits autolysosome formation. A: Diagram showing involvement of beclin1 and Rab7 in autophagosome-endosome-lysosome fusion during the formation of autolysosome. B: Hepatitis C virus (HCV)–infected Huh-7.5 cells were harvested at day 6 and day 21 and transfected with light chain 3 (LC3) green fluorescent protein (GFP). Cells were stained with DAPI (blue), antibodies against Rab7 (red), and antibodies against beclin1 (red). Co-localization of beclin1 with LC3 was examined under fluorescence microscope. C: Co-localization of Rab7 with LC3 was examined under confocal microscopy. D: Quantification of LC3 puncta in uninfected and HCV-infected Huh-7.5 cells. E: Co-localization of LC3 with Rab7 quantified by ImageJ version 1.52c. F: Co-localization of beclin1 and LC3 in uninfected, early-infected, and late-infected Huh-7.5 cells by ImageJ. G: Western blot showing the expression of Rab7, Rab5, and VPS34 in HCV-infected Huh-7.5 cells. Arrows represent LC3 GFP puncta in autophagosomes. ∗P < 0.05. Scale bars = 10 μm (B and C). Original magnification, ×60.