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. 2018 Oct;188(10):2339–2355. doi: 10.1016/j.ajpath.2018.06.022

Figure 6.

Figure 6

Beclin1 silencing and overexpression modulates autophagy flux in infected Huh-7.5 cells. A: Uninfected and persistently infected Huh-7.5 cells at day 21 were treated with Bafilomycin A for 24 hours. Cell lysates were examined for the expression of p62 and light chain 3B II (LC3-II) levels by Western blot analysis to determine the effect of persistent hepatitis C virus (HCV) replication on autophagy flux. B: Uninfected Huh-7.5 cells were treated with different concentration of beclin1 siRNA for 48 hours. Cell lysate were examined for p62 and LC3 by Western blot analysis. C: HCV-infected Huh-7.5 cells at day 21 were transfected with beclin1 cDNA (pcDNA Beclin1) for 72 hours. Cell lysate were prepared and examined for expression of p62 and LC3B-I/II by Western blotting.