Figure 4.

There is increased mRNA expression of TGF-β1, TGF-β1R, Col1a1, α-smooth muscle actin (α-SMA; ACTA2), and Fn1 in total liver, isolated cholangiocytes, and hepatic stellate cells (HSCs) from bile duct ligated (BDL) wild-type (WT) mice (ie, reduced in Sct^−/−, SR^−/−, and Sct^−/−/SR^−/− BDL mice compared with BDL WT mice). Data are from the following: i) three evaluations from three total liver samples from three different animals; ii) three evaluations from three cumulative preparations of cholangiocytes from four mice; and iii) three evaluations from three preparations of laser capture microdissection–isolated HSCs from three mice. Data are expressed as means ± SEM. ^∗P < 0.05 versus normal WT mice; ^†P < 0.05 versus BDL WT mice. Col1a1, collagen I; Fn1, fibronectin 1; TGF-β1, transforming growth factor-β1; TGF-β1R, TGF-β1 receptor.