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. 2018 Oct;188(10):2264–2280. doi: 10.1016/j.ajpath.2018.06.015

Figure 5.

Figure 5

A and B: There is increased cellular senescence [by SA-β-galactosidase (SA-β-gal) staining] and immunoreactivity for p16 (green) in bile ducts [costained for cytokeratin-19 (CK-19); red] from bile duct ligated (BDL) compared with normal wild-type (WT) mice. The increase in cell senescence observed in BDL WT mice is reduced in Sct−/−, SR−/−, and Sct−/−/SR−/− BDL mice. Insets show cholangiocyte areas in higher magnification. C: There is increased mRNA expression of p16 and p21 in cholangiocytes but decreased expression of p16 and p21 in hepatic stellate cells (HSCs) from BDL compared with normal WT mice; cellular senescence returns to values similar to those of normal WT mice in Sct−/−, SR−/−, and Sct−/−/SR−/− BDL mice compared with BDL WT mice. Data are from three evaluations from three cumulative preparations of cholangiocytes from four mice and three evaluations from three preparations of laser capture microdissection–isolated HSCs from three mice. Data are expressed as means ± SEM (C). n = 12 (C). ∗P < 0.05 versus normal WT mice; †P < 0.05 versus BDL WT mice. Scale bar = 100 μm (B). Original magnification, ×40 (A).