Figure 2.

Tissue cobalamin concentrations are reduced and plasma MMA and plasma Hcy concentrations are increased in Cd320 KO mice fed with standard mouse chow. (A) Cobalamin concentrations in plasma and tissues from male 27-week-old Cd320 KO mice (n = 7) were normalized to the values from age-matched control male mice (n = 7, brain tissue n = 6) and plotted to display mean and standard deviation (SD). Cobalamin concentrations in blood plasma and kidney did not differ from control, but all other tissues tested had significantly reduced levels of cobalamin. (Unpaired Student’s t-test, error bars represent SD). One extreme outlier value was excluded from the control brain cobalamin analysis. (B) Plasma MMA and (C) plasma Hcy concentrations were significantly elevated in both sexes of Cd320 KO mice as compared to age-matched controls in young (9–11 weeks old) and aged mice (58–66 weeks old). (Solid line denotes mean value, unpaired Student’s t-test with Welch’s correction).