Supplementary Table 5.

Theoretical Calculations Comparing the Effects of Low and High Binding Affinity Albumin on Free Circulating Levels of PGE₂ Based on Concentrations of PGE₂ and Serum Albumin Found in Healthy Patients and Patients With ACLF

Albumin at 40 g/L (∼600 μM) (Healthy)
PGE2, pg/mL	PGE2, pM	High binding affinity, Kd μM	Free PGE2, pg/mL	Low binding affinity, Kd μM	Free PGE2, pg/mL
2	5.7		0.00007		1.4
10	28.4	0.02	0.0003	200	2.5
20	56.7		0.0007		5

Albumin at 20 g/L (∼300 μM) (ACLF)
PGE2, pg/mL	PGE2, pM	Kd μM	Free PGE2, pg/mL	Kd μM	Free PGE2, pg/mL
2	5.7		0.0001		0.80
10	28.4	0.02	0.0007	200	4.0
20	56.7		0.0013		7.99

ACLF, acute-on-chronic liver failure; PGE₂, prostaglandin E₂.