FIG 1.

Ras^G12V inhibits TXNIP expression. (A) Scatter plots showing the relationship between the H-Ras gene signature and the TXNIP mRNA expression level. Each point represents a single tumor sample from the indicated cancer data set, with sample size indicated. The correlation coefficient, Pearson's r, is shown for each data set. (B and C) RT-qPCR (B) and Western blotting (C) were used to determine relative TXNIP mRNA levels (normalized to that of β-actin) and levels of the indicated proteins, respectively, in wild-type MEFs stably transduced with pBabePuro (Vector) or pBabePuro-H-Ras^G12V (Ras). (D) Western blotting was used to determine the levels of TXNIP and Sin3A proteins in MondoA KO MEFs stably transduced with pWzlBlast (Vector) or pWzlBlast-TXNIP CDS (TXNIP). (E and F) MondoA KO::TXNIP MEFs were stably transduced with pBabePuro (Vector) or pBabePuro-H-Ras^G12V (Ras), as indicated. RT-qPCR (E) and Western blotting (F) were used to determine relative TXNIP mRNA levels (normalized to that of β-actin) and the levels of the indicated proteins in each cell population. The experiments shown in panels B and E were repeated at least twice, and representative experiments are shown. Values are reported as means and SD. Statistical significance was determined using t tests. ***, P < 0.001.