FIG 5.

Analysis of insoluble protein fraction in LONP1-depleted cells. (A) Silver-stained gels obtained from the insoluble fraction of mitochondria from control and LONP1-KD-4D cell lines. (B) Silver-stained gels of proteins obtained from the insoluble fraction of mitochondria from control and LONP1-KD-6D cell lines. Bands of interest were excised from the silver-stained SDS-PAGE gel, digested with trypsin or glu-c, and analyzed by mass spectrometry (MS). (C) Analysis of the predicted MTS sequence (MitoProt II v1.101) for the indicated proteins was done using MS data obtained from the mitochondrial pellet fraction of control and LONP1-KD-4D cell lines. The shading indicates the two tryptic peptides identified within MRPL43 MTS. N/A, not available.