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Analysis of MPP and LONP1 interaction. (A) 2D-SDS-PAGE immunodetection of LONP1 and MPP in a fibroblast control line. Complex I (ND1), complex IV (COX1), and complex II (SDHA) were identified with subunit-specific antibodies (right). Numbers indicate the molecular weights of fully assembled OXPHOS complexes. (B) The MPPβ immunoprecipitation experiment was done with control mitochondria. SDS-PAGE analysis of the indicated fractions showed steady-state levels of MPPβ and LONP1.
