FIG 2.

Sequence alignment of CLN5 (performed using Clustal Omega [http://www.ebi.ac.uk/Tools/msa/clustalo/]). The red box indicates the N-glycosylation site corresponding to human N320, which is conserved among different species and at which the identified variant rs199609750 (c.A959G) exerts its effect. The green boxes indicate the six additional N-glycosylation sites conserved among different species. The blue boxes indicate the N-glycosylation site corresponding to human N401, which is not conserved in rodents. Sequences and NCBI accession numbers used in this alignment are as follows: Homo sapiens, NP_006484.1; Bos taurus, ABD83352.1; Canis lupus familiaris (C. familiaris) NP_001011556.1; Mus musculus, NP_001028414.1. Asterisks indicate positions which have a single, fully conserved residue. A colon indicates conservation between groups of strongly similar properties. A period indicates conservation between groups of weakly similar properties.