Fig. 2.

The GhU6.3.3 promoter drives strong sgRNA expression in cotton. a Diagram of CRISPR/Cas9 expression vectors: Cas9, vector lacking sgRNA cassette used as negative control; pAtU6::sgRNA, CRISPR/Cas9 construct with sgRNA-PDS driven by proAtU6-29; pGhU6::sgRNA, CRISPR/Cas9 construct with sgRNA-PDS driven by proGhU6.3.3. b Schematic diagram of Agrobacterium-mediated transient expression in cotton cotyledon with different constructs. c sgRNA expression levels determined by RT-PCR and d qPCR (n > 8, **P < 0.01, t-test)