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. 2018 Oct 3;20:219. doi: 10.1186/s13075-018-1722-9

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — IHC and IF staining showing CYLD expression in primary cultures of FLSs from OA and RA patients. (A) Representative IHC findings of synovial CYLD expression. a, × 100; b, × 400. (B) CYLD mRNA expression in FLSs from RA patients (n = 25) compared with those from OA patients (n = 15), evaluated by RT-PCR. (C) IF staining of CYLD in primary cultures of FLSs from OA and RA patients. ([A] RA-FLSs, [B] OA-FLSs; a, DAPI [blue]; b, CYLD [red]; c, merged a and b. a, b: original magnification × 400.) Data are represented as mean ± SD