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. 2018 Oct 3;20:219. doi: 10.1186/s13075-018-1722-9

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Effect of shRNA transfection on CYLD expression in RA-FLSs. We transfected RA-FLSs with sh-CYLD over 48 h. a We evaluated mRNA level of CYLD in FLSs by RT-PCR. b We detected the protein level of CYLD by Western blot. Data are represented as mean ± SD from 3 independent experiments. **P < 0.01