Fig. 3.

Overexpression of BCRC-3 up-regulates p27 expression. a Western blot analysis of the expression levels of cell cycle related proteins (p27, CDK2, CDK4, CDK6, CyclinD1, CyclinE, p21) after transfection with BCRC-3 overexpression vectors in EJ or T24 T cell lines. b The p27 mRNA expression was detected by qRT-PCR. c qRT-PCR analysis of the expression of BCRC-3 in xenograft tumors from mice (Fig. 2f). d P27 expression levels in xenograft tumors were determined by immunohistochemistry (IHC) staining and analyzed by calculating the integrated optical density per stained area (IOD/area). e The p27 mRNA expression of xenograft tumors was detected by qRT-PCR. Data are means ± SEM of 6 mice from each group. f & g Cell cycle distributions in BCRC-3 overexpression vector and shP27 co-transfected cells were presented by flow cytometry. (The results are mean ± SEM of three experiments. Student’s t-test compared the difference in b-e and g. * P < 0.01 vs. vector, vector + shNC, or vector + shP27)