Fig. 3.

Exosomal PTENP1 serve as a mediator in intercellular communication. Exosomes (Exos) isolated from the medium of 293A, J82 and EJ cells. a qRT-PCR detection of the normalized expression of PTENP1 in the medium of 293A, J82 and EJ cells treated with RNase (2 μg/ml) alone or combined with Triton X-100 (0.1%) for 20 min. b Micrographs of exos isolated from 293A (left), J82 (middle) and EJ cells (right, bars =100 nm). c Western blots of TSG101 and CD63 in exos of cell lines. d qRT-PCR detection of the fold change of PTENP1 between exos of 293A, J82 and EJ and their producer cells. e Exos of 293A cells were labeled with PKH67; green represents PKH67, and blue represents nuclear DNA staining by DAPI. J82 and EJ cells were incubated with exos derived from 293A cells for 3 h. Results are presented as mean ± SD. *P < 0.05. All of the experiments were performed in triplicate