Fig. 5.

Effect of PTENP1 overexpression and exosomal PTENP1 on tumor in vivo. PTENP1/ NC lentiviral vector was transfected into EJ cells, namely PTENP1 vector and NC, respectively. Exosomes (Exos) were isolated from 293A cells transfected with PTENP1/ NC lentiviral vector, namely PTENP1-Exos and NC-Exos, respectively. a. Burdened nude mice inoculated in NC, PTENP1-Exos, PTENP1 vector and NC-Exos. Red arrows show position of tumor. b. The xenografts from nude mice inoculated in NC, PTENP1-Exos, PTENP1 vector and NC-Exos. c. The tumor volumes were measured every two days after injection. d. The tumor weights in nude mice at the 15 day were determined. e. Detection of PTENP1 and PTEN expression in tumor tissues of nude mice treated with NC, PTENP1 vector, PTENP1-Exos and NC-Exos by qRT-PCR. f. H&E stained images and immunohistochemistry analysis of ki67 and PTEN expression in tumor tissues. Results are presented as mean ± SD. *P < 0.05