Fig. 3.

Depletion of VOPP1 induces subcellular relocalization of WWOX from the lysosomes to the Golgi apparatus. a Subcellular localization of endogenous WWOX and VOPP1 in MDA-MB-468. Cells were fixed, permeabilized, and immunostained with anti-WWOX, anti-VOPP1, and anti-GM130 (left panels), or anti-LAMP2 (right panels) antibodies as indicated followed by appropriated secondary fluor-conjugated antibodies. b The inhibition of VOPP1 expression induced the re-localization of WWOX in the Golgi apparatus. MDA-MB-468 cells were transfected with the control siRNA (Si-Ctrl) or VOPP1 siRNA (Si-VOPP1) as indicated. Three days after the transfection, cells were fixed, permeabilized, and immunostained with anti-WWOX and anti-GM130 antibodies. Cells were then counterstained with DAPI and imaged using a fluorescence microscope (original magnification, × 100)