Figure 4.

siRNA knockdown of I2PP2A and CIP2A decreased cell motility. (A) Neuroblastoma cell lines were treated with control siRNA (siNEG) or siRNA to I2PP2A (siSET), CIP2A (siCIP2A) or both (siDual) for 48 hours. (A) Cells were plated and allowed to migrate through a micropore membrane for 24 hours. Cells were fixed and stained, and number of cells were counted. Migration was significantly decreased in all cell lines with dual inhibition. siSET only affected migration in SH-EP cells. Also, SH-EP cell line was the only one not significantly affected by siCIP2A. (B) Similar to migration, treated cells were plated and allowed to invade into a Matrigel layer for 24 hours. Also similar to migration, all four cell lines had a significant decrease in invasion after dual inhibition, but only the SH-EP cell line was unaffected by siCIP2A and significantly affected by siSET. Experiments were repeated at least in triplicate and reported as mean fold change ± SEM.