Figure 5.

Inhibition of I2PP2A led to decreased neuroblastoma tumor growth. (A) SH-EP and WAC2 cells were transfected with shI2PP2A or control (shEV) plasmids. Immunoblotting confirmed target knockdown. (B) Cells (2.5 × 10⁶ in 25% Matrigel) were injected into the flanks of immunosuppressed mice, and animals were followed for tumor growth. The shI2PP2A transfected cells grew significantly smaller tumors than the empty vector (shEV) controls in both the SH-EP and WAC2 cell lines. (C) Photo of representative animals demonstrated significantly smaller tumors from the shI2PP2A transfected cells.