Figure 8.

FTY720 decreased neuroblastoma tumor growth in vivo. (A) SK-N-AS cells (2.5 × 10⁶ cells in 25% Matrigel) were injected into the right flank of 6-week-old athymic nude mice. When tumors reached an average of 100 mm³, mice were randomized to receive either 50 μl ORA-Plus (suspension vehicle, n = 7) or FTY720 10 mg/kg/day suspended in 50 μl ORA-Plus (n = 7) once daily via oral gavage. Animals treated with FTY720 (open squares) had significantly smaller tumors than those treated with vehicle alone (closed squares). (B) Mice were weighed at the beginning of the experiment and at the time of euthanasia. There was no difference in animal weights between those treated with vehicle and those treated with FTY720. (C) SK-N-BE(2) cells (2.5 × 10⁶ cells in 25% Matrigel) were injected into the right flank of 6-week-old athymic nude mice. When tumors reached an average of 100 mm³, mice were randomized to receive either 50 μl ORA-Plus (suspension vehicle, n = 5) or FTY720 10 mg/kg/day suspended in 50 μl ORA-Plus (n = 5) once daily via oral gavage. Animals treated with FTY720 (open diamonds) had significantly smaller tumors than those treated with vehicle alone (closed diamonds). (D) Mice were weighed at the beginning of the experiment and at the time of euthanasia. There was no difference in weights between those animals treated with vehicle and those treated with FTY720.