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. 2018 Apr;93(4):903–920. doi: 10.1016/j.kint.2017.11.014

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Crown Copyright © Published by International Society of Nephrology.

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

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Figure S12 — Wt1 expression is unchanged in Vehicle versus GSI-IX-treated CAGG-CreER^TM+/–; Wt1f/f transgenic mice. (A) Shown are merged micrographs of kidney sections following immunofluorescent labeling of kidney tissue sections of CAGG-CreER^TM–/–; Wt1f/f, CAGG-CreER^TM+/–; Wt1f/f, vehicle versus GSI-IX-treated CAGG-CreER^TM+/–; Wt1f/f transgenic mice with Wt1 (Alexa Fluor 594-conjugated secondary antibody). Scale bars = 50 μm. (B) Relative transcript levels of Wt1 in primary podocytes of CAGG-CreER^TM−−;Wt1^f/f (control), CAGG-CreER^TM+/−;Wt1^f/f (mutant), vehicle-treated CAGG-CreER^TM+/−;Wt1^f/f, and GSI-IX–treated CAGG-CreER^TM+/−;Wt1^f/f transgenic mice. Bars represent the mean, error bars represent the SEM. CAGG-CreER^TM−/−;Wt1^f/f versus CAGG-CreER^TM+/−;Wt1^f/f: 1.1 ± 0.2 versus 0.4 ± 0.4, *P = 0.03, Student t-test; CAGG-CreER^TM−/−;Wt1^f/f versus vehicle-treated CAGG-CreER^TM+/−;Wt1^f/f mice: 1.1 ± 0.2 versus 0.4 ± 0.2, P = 0.07, Student t-test; vehicle-treated CAGG-CreER^TM+/−;Wt1^f/f mice versus GSI-IX–treated CAGG-CreER^TM+/−;Wt1^f/f transgenic mice: 0.4 ± 0.2 versus 0.4 ± 0.3, P = 0.93, Student t-test. n.s., not significant.