Figure S9.
Terminal deoxynucleotidyltransferase–mediated 2′-deoxyuridine 5′-triphosphate nick end-labeling (TUNEL)-positive cells in glomeruli of CAGG-CreERTM+/−;Wt1f/f transgenic mice at day (D) 12 postinduction (PI). (A-D) Shown are micrographs of glomeruli of transgenic mouse kidney tissue sections labeled with TUNEL (green arrows) and counterstained with 4′,6-diamidino-2-phenylindole (DAPI) at D12 PI. Bars = 25 μm. (A) CAGG-CreERTM−/−;Wt1f/f controls display no TUNEL-positive cells in the glomerulus. (B-D) Representative micrographs for D12 PI CAGG-CreERTM−/−;Wt1f/f mutants exhibiting TUNEL-DAPI-positive cells in the glomeruli. See also Figure 2c for TUNEL-positive primary podocytes of mutant versus control mice.