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Validation of anti-Plexin C1 monoclonal antibody PE4. (a) Total proteins from PLC/PRF/5 cells transduced with shPLXNC1 and pLKO.1 empty control lentiviral particles are resolved on SDS-PAGE and Western blotting is carried out with the PE4 monoclonal antibody. Antitubulin monoclonal antibody is used for loading control. (b) Representative images from indirect immunofluorescence staining of pLKO.1-PLC/PRF/5 and shPLXNC1-PLC/PRF/5 cells with PE4 monoclonal antibody are shown.
