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Intermittent leucine deprivation increases β cell proliferation and Ngn3 expression in islets. Representative immunofluorescence staining of pancreatic sections to detect Ki67 (A) and Ngn3 (B). Scale bar, 50 μm. The nucleus was stained with Hoechst 33342. Quantitation of the images is shown in the right panel. Data are expressed as means ± SEM. ***p < 0.001.
