DC were stimulated with 0.5 μg/ml of R848, GLA, Poly I:C or a combination for 24 hours in the presence of signalling inhibitors: PDTC (NF-κB blocker), SP60 (JNK blocker), SB20 (p38 blocker) and PD98 (MEK1 blocker). Inflammatory mediators were then measured in the culture supernatant. In each graph, cells treated with TLR agonists and untreated samples are indicated. * pμ0.01 measured by two way ANOVA and post test.