DC were treated for 24 hours with R848 or GLA alone and in combination, or untreated (Negative Control, NC). Responses were also compared with untreated cells fixed at 0 hours after isolation (T0). Cells were stained and levels of surface expression of multiple surface molecules assessed by flow cytometry: HLA-DR (A), CD11c (B), CD40 (C), CD80 (D), CD86 (E) and CCR7 (F). Each graph shows the mean and SEM of the % of positive cells for each surface molecule, as indicated. ** p<0.01 measured by One way ANOVA and post test.