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. Author manuscript; available in PMC: 2019 Oct 1.
Published in final edited form as: J Immunol Methods. 2018 Aug 8;461:37–43. doi: 10.1016/j.jim.2018.08.002

Figure 3. Multiplex opsonophagocytosis assay (MOPA) to measure killing of multiple pneumococcal serotypes.

Figure 3.

On the left a typical MOPA plate well is shown, in which serum is added to a mixture of phagocytes (differentiated HL-60 cells), rabbit complement, and multiple serotypes of pneumococci, each carrying resistance to a different antibiotic. Dilutions of this mixture are plated on antibiotic-containing media (center) to select for growth of a single serotype. Bacterial colonies are enumerated using an automated counter to generate a killing curve as shown on the right (see Figure 2 for a detailed description).