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. 2018 Sep 26;9:1087. doi: 10.3389/fphar.2018.01087

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Copyright © 2018 Wang, Chen, Liu, Liu, Luo, Chen, Deng, Luo, He, Zhang, Hill, Li and Wu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Macrophage quantification in white adipose tissue. (A) Representative images of macrophages as assessed by F4/80 Immunofluorescence in eWAT from ND-WT, ND-Pai-1^−/−, HFD-WT, HFD-Pai-1^−/−, and PAI-039-treated mice. Scale bars, 100 μm. HPF: high power field. (B) Quantification of anti-F4/80 positive-macrophage infiltration of eWAT. *P < 0.05 vs. ND-WT; **P < 0.05 vs. HFD-WT. (C,D) The gene profile of the M1 (C) and M2 (D) phenotype as assessed by quantitative RT-PCR of eWAT in ND-WT, HFD-WT, HFD-Pai-1^−/−, and PAI-039-treated mice, respectively. All bars show Mean ± SEM. Data are mean of three experiments and are expressed as fold-control. *P < 0.05 vs. ND-WT; **P < 0.05 vs. HFD-WT.