Figure 1.

Schematic of how the promoter trap element functions. A) Insertion of the piggyBac promoter trap element into the TTAA piggyBac insertion site contained within an intron. B) The transcript strandedness must be consistent with that of the AmCyan ORF. C) Splicing of the promoter trap element and subsequent tetramer formation of the AmCyan protein to produce a functional AmCyan reporting fluorescent protein. Longer protein tags from the endogenous An. stephensi transcript may potentially interfere with the quaternary functional structure of AmCyan. D) Splicing of the promoter trap element containing the IRES element allowing for expression of the AmCyan protein independently of the endogenous peptide tag may alleviate potential interference of a peptide tag on the quaternary structure formation of AmCyan.