Figure 2.

Schematic of how the self-marooning piggyBac transposase element functions. A) The cassette for the piggyBac transposase self-integrates into the genome. The marooning construct is flanked by 5′ and 3′ piggyBac end and contains an internal 5′ end flanking a second fluorescent marker. B) Expression of the transposase results in the remobilization of the ECFP fluorescent marker. C) The vasa-driven piggyBac transposase construct is marooned without a 3′ piggyBac end and is identifiable by the presence of the DsRed fluorescent marker and the absence of the ECFP fluorescent marker.