Table 2. Occurrences of resolvable splicing events of the promoter trap element using RNASeq analysis and their respective insertion locations in the An. stephensi genome.
| Construct | Transcript tag* | # occurrences | Scaffold insertion | Junction location |
|---|---|---|---|---|
| Non-IRES | ASTE001888 | 1 | KB664416:466299 | 5′-UTR |
| ASTE003097 | 3 | KB664418:103665 | Intron 1 of 4 | |
| ASTE005963 | 1 | KB664733:36186 | 5′-UTR | |
| ASTE006277 | 93 | KB664587:103868 | 5′-UTR | |
| ASTE006314 | 1 | KB664288:636233 | 5′-UTR | |
| ASTE006629 | 2 | KB665199:435749 | 5′-UTR | |
| ASTE008287 | 1 | KB664477:412329 | 5′-UTR | |
| ASTE011209 | 1 | KB664576:155320 | Intron 5 of 5 | |
| IRES | ASTE000126 | 1 | KB664954:1365602 | Intron 2 of 2 |
| ASTE000270 | 1 | KB664732:782272 | 5′-UTR | |
| ASTE000780 | 4 | KB665232:679279 | 5′-UTR | |
| ASTE003737 | 1 | KB664450:281209 | Intron 2 of 2 | |
| ASTE006277 | 30 | KB664587:103868 | 5′-UTR | |
| ASTE008914 | 1 | KB664428:218959 | 5′-UTR | |
| ASTE009536 | 3 | KB664400:665982 | 5′-UTR | |
| ASTE010450 | 1 | KB664844:68028 | Intron 1 of 10 | |
| ASTE015161 | 2 | KB664456:135953 | 5′-UTR |
*
Tags were a minimum of 14 nucleotides in length and confirmed manually to the RNASeq mapped data to confirm the junction. The transcript accession number represent the An. stephensi genome strain SDA500, version 1, www.vectorbase.org.