Figure 1.

Maximum likelihood phylogeny calculated with RAxML and based on a 2,379 bp alignment of 152 subtilisin-like serine protease codon nucleotide sequences from Entomophthoromycotina that contain the peptidase S8/S53-subtilisin (PF00082) domain. Branches are colored for eachs species as Entomophthora muscae (Blue), Pandora formicae (Purple), Conidiobolus coronatus (Pink), C. thromboides (brown), C. incongruus (orange), and B. meristoporus (Green). For each SLSP, the accession number and protein domains additional to PF00082 are shown. The three identified clusters: Protease K cluster (A), Pyrolysin/osf protease cluster (B), and the new bacillopeptidase-like Entomophthorales cluster (C), are marked in the gray circle surrounding the tree and with gray background for cluster B and C.