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Platelet aggregation induced by U46619 1 μM and U46619 50 nM + epi 10 μM. Washed platelets in presence or absence of apyrase VII were pre-treated with the NADPH oxidase inhibitor apocynin 300 μM, the reactive oxygen species (ROS) scavenger EUK-134 250 μM or the intracellular calcium chelator BAPTA-AM 20 μM, respectively. Data are expressed as Mean ± SE.
