Table 3.

List of all target recognition features that were used for SVM target prediction modeling^a

Feature name	RFE rank
Seed site conservation	1
Target site location in UTR	2
GC content of target site	3
UG count	4
AG count	5
UTR length	6
Free energy of seed sequence binding	7
Pentamer motif match	8
GC content of 3′ end of target site	9
GC content of upstream nucleotides of target site	10
Position 1 A	11
Position 9 U	12
Position 1 G	13
CG count	14
Position 13 G	15
UA count	16
Position 11 G	17
AC count	18
A count	19
Position 9 C	20
Position 12 G	21
Target site <200 n.t. to UTR end	22
Position 10 AU	23
AA count	24
Position 1 AU	25
GG count	26
Position 16 G	27
GA count	28
Position 14 G	29
Position 9 A	30
Position 13 C	31
Position 9 AU	32
Position 13 AU	33
C count	34
CU count	35
AU count	36
CC count	37
Position 12 C	38
Position 11 AU	39
Position 0 G	40
Position 11 C	41
Position 15 G	42
GU count	43
Position 16 U	44
UC count	45
CA count	46
Position 16 C	47
G count	48
Target site > 800 n.t. to UTR end	49
Position 11 U	50

^aTop-ranking target recognition features were identified by SVM-RFE. The seed-binding site is designated as positions 2–8. These features were summarized by analysis of target sites in 3′-UTR sequences.