Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Sep 26;9:2190. doi: 10.3389/fimmu.2018.02190

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2018 Corapi, Carrizo, Compagno and Laderach.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Comparison of Galectin-1 expression in immune cells. Gal-1 is detected by western blot in purified immune subpopulations. T lymphocytes were purified from lymph nodes, antigen-presenting cells from spleens. The specificity of the technique was verified using Lgals1-/- cells. Additionally, Gal-1 levels in TRAMP-C1 cells are comparatively shown. 20 μg of protein was resolved on 15% SDS-PAGE and blotted with commercial anti-Gal-1 antibodies. (A) Representative immunoblot (n = 3). (B) Mean Gal-1 levels in the three independent experiments. The results are expressed as Gal-1/β-actin ratio. (C,D) Immunosuppressive effect of endogenous Gal-1 expression in TRAMP-C1 tumor cells and in lymphocytes. Lymphocytes harvested from wild type (C) or Lgals1-/- (D) mice were polyclonaly activated in presence of 10% wild type APC and 1% of control or modified TRAMP-C1 tumor cells. Proliferation was measured at 72 h by CFSE dilution. Black line: TRAMP-C1 cells with downregulated Gal-1 (stable transduced, 90% of Gal-1 down-regulation-data not shown-). Gray line: TRAMP-C1 cells transduced with a control lentivirus. Representative of two independent experiences.