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SGT-53 increases apoptosis and inhibits proliferation of 4T1 tumor in vivo. IHC staining of tumors treated as shown in Figure 3A. Representative IHC staining (A) and quantification (B) of Casp3, TUNEL, and Ki-67 are shown. Scale bar, 50 µm. At least five fields of view from three tumor sections were counted using the ImmunoRatio, an automated cell counting software (http://153.1.200.58:8080/immunoratio/). Data are shown as mean ± SEM. *p < 0.05, 1-way ANOVA with Bonferroni t-test.
