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. 2018 Aug 1;7(10):e1484982. doi: 10.1080/2162402X.2018.1484982

Figure 7.

Figure 7.

SGT-53 enhances immune responses in 4T1 tumor in vivo. Tumor cells were dissociated and immune cell infiltration was assessed via FACS. (A) Infiltrating immune cells were identified by gating CD45+ live cells including DCs (CD11c+I-A/I-E+), macrophages (CD11b+F4/80+), activated CD4 TILs (CD3+CD4+CD107a+), and activated CD8 TILs (CD3+CD8+CD107a+). Infiltrating cells are shown in terms of absolute number of cells per 1 × 104 live cells collected (n = 6–8). (B) Representative FACS plots (left panels) and graphs (right panels) of CTLs (CD3+CD8+GzmB+ or CD3+CD8+IFNγ+) are shown. Numbers in the plots indicate the percentage of cells (n = 6–8). (C) Immunosuppressive MDSCs (CD11b+Gr1+) and Tregs (CD3+CD4+FoxP3+) were identified (n = 6–8). (D) Altered expression of genes associated with immunosuppression in the tumor tissue were assessed via NanoString. (E) Expression of genes associated with immunosuppressive enzymes was assessed by RT-PCR in the tumors (n = 6–8). The fold-change relative to untreated tumors is shown. Data are shown as mean ± SEM. *p < 0.05, 1-way ANOVA with Bonferroni t-test.