Figure 3.
Combination immune checkpoint blockade is effective in an orthotopic mesothelioma model.
BALB/c mice (n = 5 PBS, n = 10 αCTLA4+ αOX40) were injected intrapleurally with 2 × 105 AB1-HA cells on day 0 and treated with PBS or ICPB on days 6, 8 and 10. (A) Overall survival. (B) Tumour burden (weight of heart + lungs + tumour) for individual mice on the day they were culled. (C) Representative histology (20 x Mag) showing haematoxylin and eosin (H&E) staining of pleural tissues taken from two mice randomly selected from each treatment group (L = lung; H = heart and T = tumour). ICPB (αC+ αO); 100 μg αCLTA-4 + 200 μg αOX40. Data shows mean ± SD. *** p < 0.001. Survival analysis: Log rank (Mantel-Cox). Tumour burden analysis: unpaired t-test (Mann-Whitney).