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. 2018 Aug 6;7(10):e1488565. doi: 10.1080/2162402X.2018.1488565

Figure 4.

Figure 4.

AKT-inhibition is associated with increased glycolysis. CD8+ TN cells were stimulated with allo-mDCs in presence of DMSO (Ctrl) or AktiVIII (12 µM), MK (5 µM), GDC (10 µM), GSK1 (2.5 µM) or GSK2 (10 µM) and (A-C) challenged in different metabolic assays or (D) followed by gene analysis. (A) Mitochondrial respiration and glycolytic function of one representative out of 4 donors, n = 4–6. (B) Glycolysis in 4 independent donor, different symbols depict different donors. (C) Association between glycolysis and CD62L expression (Median Fluorescence Intensity, MFI). Data of all Ctrl and AKT-inhibited conditions included. (D) Fold change of PFKM and CPT1A expression compared to Ctrl cells. Statistical analysis was performed using (B) a two-tailed paired t-tests or (C) linear regression with 95% confidence interval, Median Fluorescence Intensity (MFI), ǂp < 0.001, #p < 0.01, *p < 0.05.