Table 2. Comparison the reactivity of folded rtFhSAP-2, rtFhFtn-1and rFhLAP with their unfolded forms by ELISA.
| Concentrations of proteins (μg/ml) | rtFhSAP2 | rtFhFtn-1 | rFhLAP | |||
|---|---|---|---|---|---|---|
| Refold | Unfold | Refold | Unfold | Refold | Unfold | |
| 8 | 2.006 | 1.128 | 1.243 | 0.948 | 1.788 | 0.975 |
| 4 | 2.102 | 1.139 | 1.127 | 0.873 | 1.747 | 0.954 |
| 2 | 2.042 | 1.299 | 1.329 | 0.761 | 1.645 | 0.931 |
| 1 | 1.938 | 1.056 | 0.988 | 0.611 | 1.332 | 0.719 |
| 0.5 | 1.961 | 0.964 | 0.712 | 0.401 | 0.890 | 0.389 |
| 0.25 | 2.016 | 1.371 | 0.764 | 0.317 | 0.791 | 0.362 |
Recombinant proteins samples were assessed in triplicate in defined concentrations.
The difference between negative samples (refold and unfold forms) were narrow.