Table 2. Thermostability of chloroform extract of BRARP.
List of microorganisms |
Chloroform extract at 95°C at different time periods (Min); Zone of inhibition (mm) | |||||
---|---|---|---|---|---|---|
5 | 15 | 30 | 45 | 60 | 90 | |
Gram-negative bacteria | ||||||
494 (Isolate) | 11.00 ± 0.01b | 11.00 ± 0.03b | 10.50 ± 0.05bc | 12.00 ± 0.01a | 11.00 ± 0.04b | 11.00 ± 0.03b |
ATCC 35150 | 13.00 ± 0.02a | 12.00 ± 0.01b | 11.00 ± 0.03c | 13.00 ± 0.02a | 10.00 ± 0.03d | 12.00 ± 0.01b |
ATCC 43894 | 12.00 ± 0.02b | 14.00 ± 0.03a | 10.00 ± 0.03c | 12.00 ± 0.02b | 12.00 ± 0.05b | 14.00 ± 0.03a |
Gram-positive bacteria | ||||||
ATCC 13150 | 10.50 ± 0.01a | 09.50 ± 0.03b | 08.00 ± 0.02d | 10.50 ± 0.01a | 09.00 ± 0.05c | 09.50 ± 0.03b |
KCTC 21004 | 10.00 ± 0.02a | 09.00 ± 0.03b | 09.00 ± 0.01b | 10.00 ± 0.02a | 09.00 ± 0.03b | 09.00 ± 0.03b |
KCTC 3545 | 10.00 ± 0.02ab | 10.30 ± 0.05a | 10.00 ± 0.02ab | 10.00 ± 0.02ab | 10.00 ± 0.03ab | 10.30 ± 0.05a |
KCTC 13302 | 10.00± 0.02a | 09.00± 0.03b | 10.00± 0.03a | 10.00 ± 0.02a | 10.00± 0.05a | 09.00 ± 0.03b |
Fungi | ||||||
KCTC 7965 | 10.00 ± 0.03a | 09.00 ± 0.01b | 10.00 ± 0.03a | 10.00 ± 0.03a | 10.00 ± 0.04a | 09.00 ± 0.01b |
KCTC 6145 | 09.00 ± 0.02 | 08.00 ± 0.03 | 08.30 ± 0.03 | 09.00 ± 0.02 | 09.00 ± 0.03 | 08.00 ± 0.03 |
KCTC 6143 | - | - | - | - | - | - |
KCTC 6317 | 12.30 ± 0.02a | 12.30 ± 0.03a | 12.00 ± 0.01ab | 12.30 ± 0.02a | 12.00 ± 0.04ab | 12.30 ± 0.03a |
-: not active
a: more sensitive
b: moderate sensitive
c: less sensitive, MHA-Muller Hinton Agar, MRS-De Man, Rogosa and Sharpe agar