Fig 3. RNA Pol I inhibitor, CX-5461, disrupts consolidation of long-term memory, but not learning.
A) Timeline of the training protocol. Note that half of the animals were euthanized 1 h after the third training trial for qPCR analysis (see 3D) while the remainder were given a retention test 24 h later (see 3C). B) Representative paths (trajectories) of animals during pre-training, training and the 10 min retention test given 24 h after the 3rd training trial (grey tracings). Red circles indicate the animal’s location when a shock was received. Grey circles indicate the position of the animal where it would have received a shock had the shock zone been active. C) Comparison of learning and memory between APA trained mice who received intracranial injection of vehicle (black circles) and CX-5461 (grey circles). Left, Number of entrances during the 30 min trials. No significant difference in the number of entrances indicates equivalent learning of CX-5461 treated animals and vehicle-treated controls. Right, Time to first entry the shock zone during each training trial and on the 24 h retention test. There were no significant differences in between-trial memory or between the two groups during training. In contrast, the vehicle injected animals continue to show place avoidance memory 24 h later; whereas CX-5461 injected animals do not. D) Real-Time qPCR relative expression shows that CX-5461 has no effect on Pol II dependent c-fos and c-jun transcription, but does inhibit Pol I dependent rRNA transcription. *p<0.05, **p<0.01.