Table 2. Genes that show significant and nominal evidence of recent selection in D. melanogaster and in D. simulans.
D. melanogaster | ||||||||||||||||||
Gene ID | Gene name | Function | θw | TajD | TajD pval | TajD pval corrected | nFWH | nFWH pval | nFWH pval corrected | EW | EW pval | EW pval corrected | DHEW pval | DHEW pval corrected | TajD rank | TajD rank against null | nFWH rank | nFWH rank against null |
FBgn0039636 | Atg14 | Autophagy: Nucleation | 9.1430 | -1.9260 | 0.0036 | 0.0479 | -1.8983 | 0.0416 | 0.4079 | 0.0599 | 0.0063 | 0.0134 | 0.0020 | 0.0781 | 1.47 | 0.41 | 4.41 | 3.27 |
FBgn0260858 | Ykt6 | Autophagy/Phagocytosis: Phagosome internalization; autophagosome expansion/fusion with lysosome | 2.8690 | -2.1510 | 0.0012 | 0.0271 | -0.6169 | 0.1664 | 0.4079 | 0.5516 | 0.0003 | 0.0013 | 0.0270 | 0.0782 | 5.88 | 6.94 | 45.59 | 51.02 |
FBgn0039705 | Atg16 | Autophagy/Phagocytosis: Phagosome maturation; autophagosome expansion | 5.9160 | -1.2470 | 0.0814 | 0.1579 | -1.7148 | 0.0552 | 0.4079 | 0.1303 | 0.0000 | 0.0000 | 0.0100 | 0.0781 | 80.88 | 77.96 | 7.35 | 4.90 |
FBgn0010435 | emp | Phagocytosis: Recognition—receptor | 4.4820 | -1.1770 | 0.1058 | 0.1811 | -1.5438 | 0.0656 | 0.4079 | 0.2024 | 0.0132 | 0.0241 | 0.0160 | 0.0781 | 92.65 | 89.39 | 5.88 | 3.67 |
FBgn0014011 | Rac2 | Phagocytosis: Internalization | 5.3780 | -0.8280 | 0.2168 | 0.2634 | -1.5064 | 0.0666 | 0.4079 | 0.0452 | 0.0435 | 0.0662 | 0.0280 | 0.0782 | 60.29 | 60.41 | 8.82 | 5.71 |
FBgn0031969 | pes | Phagocytosis: Recognition—receptor | 15.4180 | -1.2590 | 0.0820 | 0.1584 | -0.1963 | 0.2664 | 0.4152 | 0.0143 | 0.0019 | 0.0051 | 0.0300 | 0.0782 | 4.41 | 5.71 | 76.47 | 76.33 |
D. simulans | ||||||||||||||||||
Gene ID | Gene name | Function | θw | TajD | TajD pval | TajD pval corrected | nFWH | nFWH pval | nFWH pval corrected | EW | EW pval | EW pval corrected | DHEW pval | DHEW pval corrected | TajD rank | TajD rank against null | nFWH rank | nFWH rank against null |
FBgn0191479 | Rab1 | Autophagy/Phagocytosis: Fusion with lysosome | 1.5723 | -1.4054 | 0.0766 | 0.2167 | -0.8680 | 0.1365 | 0.7198 | 0.3673 | 0.0922 | 1.0000 | 0.0078 | 0.8734 | 12.31 | 11.64 | 6.15 | 3.02 |
FBgn0191695 | Atg8b | Autophagy/Phagocytosis: Phagocytosis internalization; autophagosome expansion | 2.8301 | -1.6322 | 0.0405 | 0.2167 | -0.8833 | 0.1246 | 0.7198 | 0.2857 | 0.0183 | 1.0000 | 0.0117 | 0.8734 | 10.77 | 11.64 | 1.54 | 2.16 |
FBgn0181823 | Vamp7 | Autophagy/Phagocytosis: Fusion with lysosome | 1.8867 | -1.9589 | 0.0222 | 0.2167 | -0.7603 | 0.1454 | 0.7198 | 0.4388 | 0.0242 | 1.0000 | 0.0197 | 0.8734 | 1.54 | 0.86 | 7.69 | 3.02 |
FBgn0185198 | Rac1 | Phagocytosis: Internalization | 2.8301 | -1.9303 | 0.0186 | 0.2167 | -0.3022 | 0.2352 | 0.7198 | 0.2347 | 0.0930 | 1.0000 | 0.0450 | 0.8734 | 6.15 | 6.47 | 26.15 | 22.41 |
θw, Watterson’s θ; TajD, Tajima’s D; nFWH, normalized Fay and Wu’s H; EW, Ewens-Watterson statistic; DHEW, DHEW compound statistic P-value; TajD/nFWH/EW/DHEW pval corrected, P-values with multiple testing correction. For each focal-control pair, comparison score was calculated for Tajima’s D and Fay and Wu’s H and the scores were ranked from largest to smallest in the ‘rank’ column. Random assignment of a control gene to be “focal” and re-calculating the ranks of comparison scores created a distribution of ranks. The ranks of comparison scores from true coupling of focal and control genes compared to this distribution are listed in the ‘rank against null’ column.