Figure 6. YES controls multiple cell cycle regulators.

A, Western blot analysis of SFK members in the indicated cell lines. B, Western blot analysis of Lats-YAP signaling in parental and YES-KO HeLa cells. C, Total cell lysates from parental and YES-KO HeLa cells were subjected to Western blotting analysis with various cell cycle regulators. D, Total cell lysates from the indicated HeLa cells were subjected to Western blotting analysis. E, YES deletion resulted in no significant DNA damage. The positive controls (first lane) are HeLa cells treated with 5-FU (Fluorouracil) or Doxorubicin. F, Expression profiling of cell cycle regulators identified multiple genes that are regulated by YES. The RT² cell cycle arrays (Qiagen) were used and experiments were performed following the manufacturer’s instructions. F.C.: fold change. G, Quantitative RT-PCR for p21 and RBL1 in indicated HeLa cell lines. Data are expressed as the mean ± s.e.m. of three independent experiments. ^***: p< 0.001; *: p< 0.05 (t-test).