Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Cell line (Mus musculus) |
L Cells | American Type Culture Collection |
Cat #: CRL-2648; RRID:CVCL_4536 |
PMID:14056989 |
| Cell line (Mus musculus) |
L Wnt3A Cells | American Type Culture Collection |
Cat #: CRL-2647; RRID:CVCL_0635 |
PMID:12717451 |
| Cell line (Homo sapiens) |
HeLa-MZ | other | Clone of HeLa (American Type Culture Collection Cat#: CCL-2) provided by Prof. Lucas Pelkmans (University of Zurich) |
|
| Cell line (Homo sapiens) |
CRISPR DDIT3 | this paper | ||
| Cell line (Homo sapiens) |
CRISPR TFAP2A | this paper | ||
| Transfected construct (Homo sapiens) |
GSK3B | Addgene | Cat #: 49491 | |
| Transfected construct (Homo sapiens) |
GSK3BS9A | Addgene | Cat #: 49492 | |
| Transfected construct (Homo sapiens) |
Wnt Project plasmid library |
Addgene | Kit # 1000000022 | |
| Transfected construct (Homo sapiens) |
pCMV-SREBP-1a(460 ) |
American Type Culture Collection |
Cat #: 99637 | PMID:9062341 |
| Transfected construct (Homo sapiens) |
pCMV-SREBP-1c(436 ) |
American Type Culture Collection |
Cat #: 99636 | PMID:9062341 |
| Transfected construct (Homo sapiens) |
pCMV-SREBP-2(468) | American Type Culture Collection |
Cat #: 63452 | PMID:9062341 |
| Transfected construct (Homo sapiens) |
DDIT3-mCherry | this paper | ||
| Transfected construct (Homo sapiens) |
mCherry-TFAP2A | this paper | ||
| Transfected construct (Homo sapiens) |
mCherry-TFAP2B | this paper | ||
| Transfected construct (Homo sapiens) |
mCherry-TFAP2C | this paper | ||
| Transfected construct (Homo sapiens) |
V5-TFAP2A | this paper | ||
| Biological sample (Bos taurus) |
Lipoprotein-depleted serum | PMID:13252080 | ||
| Antibody | Rabbit anti-AP2 alpha; anti-TFAP2A |
Abcam | Cat #: ab52222 | |
| Antibody | Mouse anti-CHOP (L63F7); anti-DDIT3 |
Cell Signaling | Cat #: 2895 | |
| Antibody | Mouse anti-V5 Tag | ThermoFisher Scientific |
Cat #: R960-25 | |
| Recombinant DNA reagent |
CRISPR Forward: DDIT3 |
Microsynth | CACCGGCACCTATATCTCATCCCC | |
| Recombinant DNA reagent |
CRISPR Forward: TFAP2A |
Microsynth | CACCGGAGTAAGGATCTTGCGACT | |
| Recombinant DNA reagent |
CRISPR Reverse: DDIT3 |
Microsynth | AAACGACTGATCCAACTGCAGAGAC | |
| Recombinant DNA reagent |
CRISPR Reverse: TFAP2A |
Microsynth | AAACAGTCGCAAGATCCTTACTCC | |
| Recombinant DNA reagent |
Primer Forward: ACSL3 |
Microsynth | TGAGCTCTCTTTGCTTGGTG | |
| Recombinant DNA reagent |
Primer Forward: ACSL4 |
Microsynth | AAGGACATCCCGAAACACAC | |
| Recombinant DNA reagent |
Primer Forward: AGPAT2 |
Microsynth | GGCCTAAGGCAAAAGGATGTG | |
| Recombinant DNA reagent |
Primer Forward: AGPAT3 |
Microsynth | ACCCAAGCTCAGCAAGTCC | |
| Recombinant DNA reagent |
Primer Forward: CTCF |
Microsynth | GCCAGTCCAACCGGCTTATG | |
| Recombinant DNA reagent |
Primer Forward: LPCAT2 |
Microsynth | AGGGGAAGTGGTTGCTCAATG | |
| Recombinant DNA reagent |
Primer Forward: MGLL |
Microsynth | GAACCCAGCTCAGTTCAGG | |
| Recombinant DNA reagent |
Primer Forward: PLIN3 |
Microsynth | TTTGGCAGAGGTGGCAAAC | |
| Recombinant DNA reagent |
Primer Forward: PLIN4 |
Microsynth | AACCTGCAGGGAAGGTGTTC | |
| Recombinant DNA reagent |
Primer Forward: PNPLA2 |
Microsynth | TGGCTTCCCTAACTCAGCTTG | |
| Recombinant DNA reagent |
Primer Forward: PNPLA3 |
Microsynth | TGTCAAGGAAAACAGAAGGAAGC | |
| Recombinant DNA reagent |
Primer Reverse: ACSL3 |
Microsynth | TGAAAGGTTGCCTTCCTGAG | |
| Recombinant DNA reagent |
Primer Reverse: ACSL4 |
Microsynth | TCGCCTCAAGTTGTTGCTC | |
| Recombinant DNA reagent |
Primer Reverse: AGPAT2 |
Microsynth | CTTCAAATGAATGGGGAACTGC | |
| Recombinant DNA reagent |
Primer Reverse: AGPAT3 |
Microsynth | GCCCGGTACCTTGTGTGAC | |
| Recombinant DNA reagent |
Primer Reverse: CTCF |
Microsynth | GGTTCTCCCAAGCAGGAGCA | |
| Recombinant DNA reagent |
Primer Reverse: LPCAT2 |
Microsynth | TCTATGAACCTCGGTTGCCTTC | |
| Recombinant DNA reagent |
Primer Reverse: MGLL |
Microsynth | CAGCCACGCACTCCTCTC | |
| Recombinant DNA reagent |
Primer Reverse: PLIN3 |
Microsynth | GATCCACAGGAAGTTCAAGCTG | |
| Recombinant DNA reagent |
Primer Reverse: PLIN4 |
Microsynth | TTCCTCCTTCGCTTGCTTC | |
| Recombinant DNA reagent |
Primer Reverse: PNPLA2 |
Microsynth | TCATCTCTGGACCTAGCTGTTGC | |
| Recombinant DNA reagent |
Primer Reverse: PNPLA3 |
Microsynth | GCAGCGACTCGAGAGAAAGC | |
| Recombinant DNA reagent |
Primer set: ACSL3 |
QIAGEN | Cat #: QT01068333 | |
| Recombinant DNA reagent |
Primer set: ACTB |
QIAGEN | Cat #: QT01136772 | |
| Recombinant DNA reagent |
Primer set: AGPAT2 |
QIAGEN | Cat #: QT00104888 | |
| Recombinant DNA reagent |
Primer set: AGPAT3 |
QIAGEN | Cat #: QT00131481 | |
| Recombinant DNA reagent |
Primer set: DDIT3 |
QIAGEN | Cat #: QT01749748 | |
| Recombinant DNA reagent |
Primer set: DGAT2 |
QIAGEN | Cat #: QT00134477 | |
| Recombinant DNA reagent |
Primer set: HMGCR |
QIAGEN | Cat #: QT00004081 | |
| Recombinant DNA reagent |
Primer set: LDLR |
QIAGEN | Cat #: QT00045864 | |
| Recombinant DNA reagent |
Primer set: MGLL |
QIAGEN | Cat #: QT01163428 | |
| Recombinant DNA reagent |
Primer set: PLIN4 |
QIAGEN | Cat #; QT00112301 | |
| Recombinant DNA reagent |
Primer set: PNPLA2 |
QIAGEN | Cat #: QT00111846 | |
| Recombinant DNA reagent |
Primer set: SOAT1 |
QIAGEN | Cat #: QT01046472 | |
| Recombinant DNA reagent |
Primer set: SREBPF1 |
QIAGEN | Cat #: QT00167055 | |
| Recombinant DNA reagent |
Primer set: SREBPF1 |
QIAGEN | Cat #: QT00036897 | |
| Recombinant DNA reagent |
Primer set: SREBPF2 |
QIAGEN | Cat #: QT00255204 | |
| Recombinant DNA reagent |
Primer set: SREBPF2 |
QIAGEN | Cat #: QT00052052 | |
| Recombinant DNA reagent |
Primer set: TFAP2A |
QIAGEN | Cat #: QT00085225 | |
| Recombinant DNA reagent |
Primer set: TFAP2C |
QIAGEN | Cat #: QT00073073 | |
| Sequence-based reagent |
siRNA DDIT3 | Dharmacon | Cat #: J-062068 | |
| Sequence-based reagent |
siRNA TFAP2A | Dharmacon | Cat #: J-062799 | |
| Sequence-based reagent |
siRNA TFAP2C | Dharmacon | Cat #: J-048594 | |
| Sequence-based reagent |
siRNA APC | QIAGEN | Cat #: S102757251 | |
| Sequence-based reagent |
siRNA DGAT1 | QIAGEN | Cat #: S100978278 | |
| Sequence-based reagent |
siRNA DGAT2 | QIAGEN | Cat #: S100978278 | |
| Sequence-based reagent |
siRNA GSK3B | QIAGEN | Cat #: S100300335 | |
| Sequence-based reagent |
siRNA SOAT1 | QIAGEN | Cat #: S101428924 | |
| Commercial assay or kit |
Bio-Rad Protein Assay Kit |
Bio-Rad Laboratories | Cat #: 500 – 0006 |
|
| Commercial assay or kit |
SsoAdvanced SYBR Green Supermix |
Bio-Rad Laboratories | Cat #: 1725270 | |
| Commercial assay or kit |
Triglyceride Colorimetric Assay Kit |
Cayman Chemicals | Cat #: 10010303 | |
| Commercial assay or kit |
Wizard SV gel and PCR Clean -up system |
Promega | Cat #: A9281 | |
| Commercial assay or kit |
RNeasy Mini Kit | QIAGEN | Cat #: 74104 | |
| Commercial assay or kit |
RNeasy Mini Kit | QIAGEN | Cat #: 74104 | |
| Commercial assay or kit |
Amplex Red Cholesterol Assay Kit |
ThermoFisher Scientific | Cat #: A12216 | |
| Commercial assay or kit |
SuperScript VILO cDNA Synthesis Kit |
ThermoFisher Scientific | Cat #: 11754050 | |
| Chemical compound, drug |
Wnt Pathway Library |
Enzo Life Sciences | Cat #: BML-2838 | |
| Chemical compound, drug |
BODIPY 493/503 | ThermoFisher Scientific | Cat #: D3922 | |
| Chemical compound, drug |
Hoechst 33342 | ThermoFisher Scientific | Cat #: H3570 | |
| Chemical compound, drug |
Lipofectamine 3000 | ThermoFisher Scientific | Cat #: L3000015 | |
| Chemical compound, drug |
Lipofectamine LTX | ThermoFisher Scientific | Cat #: A12621 | |
| Chemical compound, drug |
Lipofectamine RNAiMax |
ThermoFisher Scientific | Cat #: 13778100 | |
| Chemical compound, drug |
A-922500 | Tocris Bioscience | Cat #: 3587 | PMID:18183944 |
| Chemical compound, drug |
PF-429242 | Tocris Bioscience | Cat #: 3354 | PMID:17583500 |
| Chemical compound, drug |
Torin-2 | Tocris Bioscience | Cat #: 4248 | PMID:21322566 |