(A) Schematic of the in vitro co-culture and supernatant transfer systems.
(B) Supernatant IFNγ and IL-10 detected 24, 48, and 72 hr after NK cell co-culture with L1S+LPS-stimulated or Lm-infected B6.Il10−/− BMDCs (n = 3 independent experiments pooled).
(C) Intracellular IFNγ produced by NK1.1+CD3− cells 24 hr and 72 hr after co-culture (representative of n = 3 experiments).
(D and E) Supernatant cytokines detected 24 hr (IFNγ, D) or 72 hr (IL-10, E) following NK cell exposure to L1S+LPS-stimulated B6.Il10−/− BMDCs in co-culture with or without separation with a 0.4 μM transwell insert or exposed to filtered supernatants collected 1 hr post-stimulation from BMDCs, as indicated (n = 3 independent experiments pooled).
Data are displayed as mean ± SEM; *p < 0.05 and ***p < 0.001 as measured by t test.