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. Author manuscript; available in PMC: 2018 Oct 3.
Published in final edited form as: Cell Rep. 2018 May 29;23(9):2582–2594. doi: 10.1016/j.celrep.2018.04.106

Figure 1. Soluble Factors from BMDCs Suffice to License NK Cell IL-10 Production.

Figure 1

(A) Schematic of the in vitro co-culture and supernatant transfer systems.

(B) Supernatant IFNγ and IL-10 detected 24, 48, and 72 hr after NK cell co-culture with L1S+LPS-stimulated or Lm-infected B6.Il10−/− BMDCs (n = 3 independent experiments pooled).

(C) Intracellular IFNγ produced by NK1.1+CD3− cells 24 hr and 72 hr after co-culture (representative of n = 3 experiments).

(D and E) Supernatant cytokines detected 24 hr (IFNγ, D) or 72 hr (IL-10, E) following NK cell exposure to L1S+LPS-stimulated B6.Il10−/− BMDCs in co-culture with or without separation with a 0.4 μM transwell insert or exposed to filtered supernatants collected 1 hr post-stimulation from BMDCs, as indicated (n = 3 independent experiments pooled).

Data are displayed as mean ± SEM; *p < 0.05 and ***p < 0.001 as measured by t test.