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. Author manuscript; available in PMC: 2018 Oct 3.
Published in final edited form as: Cell Rep. 2018 May 29;23(9):2582–2594. doi: 10.1016/j.celrep.2018.04.106

Figure 3. NLRP3 Regulates IL-18 Release Required for NK Cell IL-10 Production in Response to Lm or L1S+LPS.

Figure 3

(A and B) Supernatant IL-18 detected at the indicated times post-stimulation with L1S+LPS (A) or infection with WT or Δp60 Lm (B) of B6 or B6.Nlrp3−/− BMDCs (n = 3 independent experiments pooled).

(C) Serum IL-10 and IL-18 detected in uninfected (naive) or Lm-infected (104 i.v.) B6 mice at the indicated time points.

(D) Serum IL-18 detected in B6 or B6.Nlrp3−/− mice sacrificed 72 hpi.

(E and F) Serum IL-10 (E) and Lm burdens per organ (F) from B6 or B6.Il18−/− mice sacrificed 72 hpi (n = 3 independent experiments pooled with 3–5 mice per group for in vivo experiments).

(G and H) Supernatant IL-10 detected in NK cell cultures 72 hr after exposure to filtered supernatants from 1 hr L1S+LPS stimulation (G) or WT or Δp60 Lm infection (H) of B6 or B6.Il18−/− BMDCs with or without 50 pg/mL rIL-18 added to NK cell cultures.

(I and J) Supernatant IL-10 detected in NK cell cultures 72 hr after exposure to filtered supernatants from 1 hr L1S+LPS stimulation (I) or WT or Δp60 Lm infection (J) of B6 or B6.Nlrp3−/− BMDCs + 50 pg/mL rIL-18 added to NK cell cultures (n = 3 independent experiments pooled for in vitro experiments).

Data are displayed as mean ± SEM; *p < 0.05, **p < 0.01, and ***p < 0.001 as measured by t test.