(A and B) Supernatant IL-18 detected at the indicated times post-stimulation with L1S+LPS (A) or infection with WT or Δp60 Lm (B) of B6 or B6.Nlrp3−/− BMDCs (n = 3 independent experiments pooled).
(C) Serum IL-10 and IL-18 detected in uninfected (naive) or Lm-infected (104 i.v.) B6 mice at the indicated time points.
(D) Serum IL-18 detected in B6 or B6.Nlrp3−/− mice sacrificed 72 hpi.
(E and F) Serum IL-10 (E) and Lm burdens per organ (F) from B6 or B6.Il18−/− mice sacrificed 72 hpi (n = 3 independent experiments pooled with 3–5 mice per group for in vivo experiments).
(G and H) Supernatant IL-10 detected in NK cell cultures 72 hr after exposure to filtered supernatants from 1 hr L1S+LPS stimulation (G) or WT or Δp60 Lm infection (H) of B6 or B6.Il18−/− BMDCs with or without 50 pg/mL rIL-18 added to NK cell cultures.
(I and J) Supernatant IL-10 detected in NK cell cultures 72 hr after exposure to filtered supernatants from 1 hr L1S+LPS stimulation (I) or WT or Δp60 Lm infection (J) of B6 or B6.Nlrp3−/− BMDCs + 50 pg/mL rIL-18 added to NK cell cultures (n = 3 independent experiments pooled for in vitro experiments).
Data are displayed as mean ± SEM; *p < 0.05, **p < 0.01, and ***p < 0.001 as measured by t test.